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endogenous control gene human gapdh  (Thermo Fisher)


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    Thermo Fisher endogenous control gene human gapdh
    Endogenous Control Gene Human Gapdh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endogenous+control+gene+human+gapdh/Phosphate/us12447173-298-46-73
    Average 99 stars, based on 1 article reviews
    endogenous control gene human gapdh - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Reduced PLCG1 expression is associated with inferior survival for myelodysplastic syndromes
    Article Snippet: .. Real‐time RT‐PCR was performed to quantify the expression level of PLCG1 by the TaqMan probe method (Applied Biosystems) using cDNA as template with co‐amplification of an endogenous control gene human GAPDH (Applied Biosystems). ..

    Expressing:

    Article Title: Reduced PLCG1 expression is associated with inferior survival for myelodysplastic syndromes
    Article Snippet: .. Real‐time RT‐PCR was performed to quantify the expression level of PLCG1 by the TaqMan probe method (Applied Biosystems) using cDNA as template with co‐amplification of an endogenous control gene human GAPDH (Applied Biosystems). ..

    Control:

    Article Title: Reduced PLCG1 expression is associated with inferior survival for myelodysplastic syndromes
    Article Snippet: .. Real‐time RT‐PCR was performed to quantify the expression level of PLCG1 by the TaqMan probe method (Applied Biosystems) using cDNA as template with co‐amplification of an endogenous control gene human GAPDH (Applied Biosystems). ..

    Article Title: Pharmaceutical composition containing double-stranded ribonucleic acid inhibiting expression of complement C5
    Article Snippet: From the cultured cells, a template lysate for real-time PCR was prepared by using a CellAmp (registered trademark) Direct RNA Prep Kit for RT-PCR (Real Time) (from Takara Bio Inc., catalog number: 3732) and Proteinase K (from Takara Bio Inc., catalog number: 9034) in accordance with a protocol provided by Takara Bio Inc. .. Thereafter, cDNA was prepared by using a PrimeScript (registered trademark) RT Master Mix (Perfect Real Time) (from Takara Bio Inc., catalog number: RR036A) in accordance with a protocol provided by Takara Bio Inc. Further, Ct values were measured for the target gene human C5 and the endogenous control gene human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) by using an EagleTaq Universal Master Mix (ROX) (from Roche Diagnostics K.K., catalog number: 07260296190) and a TaqMan probe (from Applied Biosystems, C5: Hs00156197_m1; GAPDH: Hs02758991_g1) with an ABI7900HT real-time PCR system (from Applied Biosystems) in accordance with a protocol provided by Applied Biosystems. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Pharmaceutical composition containing double-stranded ribonucleic acid inhibiting expression of complement C5
    Article Snippet: From the cultured cells, a template lysate for real-time PCR was prepared by using a CellAmp (registered trademark) Direct RNA Prep Kit for RT-PCR (Real Time) (from Takara Bio Inc., catalog number: 3732) and Proteinase K (from Takara Bio Inc., catalog number: 9034) in accordance with a protocol provided by Takara Bio Inc. .. Thereafter, cDNA was prepared by using a PrimeScript (registered trademark) RT Master Mix (Perfect Real Time) (from Takara Bio Inc., catalog number: RR036A) in accordance with a protocol provided by Takara Bio Inc. Further, Ct values were measured for the target gene human C5 and the endogenous control gene human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) by using an EagleTaq Universal Master Mix (ROX) (from Roche Diagnostics K.K., catalog number: 07260296190) and a TaqMan probe (from Applied Biosystems, C5: Hs00156197_m1; GAPDH: Hs02758991_g1) with an ABI7900HT real-time PCR system (from Applied Biosystems) in accordance with a protocol provided by Applied Biosystems. ..



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    taqman ® gene expression assays for human gapdh endogenous control  (Thermo Fisher)
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    Expression levels of ADH1 in differentiating human subcutaneous pre-adipocytes. ( A ) Representative composite image of immunoblot analysis. Total protein was isolated at the indicated time points during differentiation of human subcutaneous pre-adipocytes (Day 0) to cultured mature adipocytes (Day 14), resolved by SDS-PAGE, and immunoblotted with antibodies specific for the three ADH1 isoforms (ADH1A, ADH1B, <t>ADH1C);</t> the adipokines FABP4, adiponectin, PPARγ, and C/EBPα; and the adipose-specific glucose transporter GLUT4. β-Actin was used as a loading control. Full length blots are presented in Supplementary Figures and . ( B ) Quantitative analysis of ADH1B protein expression. Western blot analysis was performed at least three times. Fluorescent signal intensity was quantified and normalized to β-Actin control. Data is presented as the mean ± s.e.m. AU, Arbitrary Units.
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    Image Search Results


    Expression levels of ADH1 in differentiating human subcutaneous pre-adipocytes. ( A ) Representative composite image of immunoblot analysis. Total protein was isolated at the indicated time points during differentiation of human subcutaneous pre-adipocytes (Day 0) to cultured mature adipocytes (Day 14), resolved by SDS-PAGE, and immunoblotted with antibodies specific for the three ADH1 isoforms (ADH1A, ADH1B, ADH1C); the adipokines FABP4, adiponectin, PPARγ, and C/EBPα; and the adipose-specific glucose transporter GLUT4. β-Actin was used as a loading control. Full length blots are presented in Supplementary Figures and . ( B ) Quantitative analysis of ADH1B protein expression. Western blot analysis was performed at least three times. Fluorescent signal intensity was quantified and normalized to β-Actin control. Data is presented as the mean ± s.e.m. AU, Arbitrary Units.

    Journal: Scientific Reports

    Article Title: Further evidence supporting a potential role for ADH1B in obesity

    doi: 10.1038/s41598-020-80563-z

    Figure Lengend Snippet: Expression levels of ADH1 in differentiating human subcutaneous pre-adipocytes. ( A ) Representative composite image of immunoblot analysis. Total protein was isolated at the indicated time points during differentiation of human subcutaneous pre-adipocytes (Day 0) to cultured mature adipocytes (Day 14), resolved by SDS-PAGE, and immunoblotted with antibodies specific for the three ADH1 isoforms (ADH1A, ADH1B, ADH1C); the adipokines FABP4, adiponectin, PPARγ, and C/EBPα; and the adipose-specific glucose transporter GLUT4. β-Actin was used as a loading control. Full length blots are presented in Supplementary Figures and . ( B ) Quantitative analysis of ADH1B protein expression. Western blot analysis was performed at least three times. Fluorescent signal intensity was quantified and normalized to β-Actin control. Data is presented as the mean ± s.e.m. AU, Arbitrary Units.

    Article Snippet: RNA concentrations were measured on a NanoDropTM spectrophotometer and qPCR was performed using TaqMan ® Gene Expression Assays for human ADH1A , ADH1B , ADH1C , and GAPDH endogenous control (ThermoFisher Scientific, Waltham, MA). qPCR analysis was performed in triplicate or quadruplicate.

    Techniques: Expressing, Western Blot, Isolation, Cell Culture, SDS Page, Control